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KMID : 0363720060390060453
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Korean Journal of Anatomy
2006 Volume.39 No. 6 p.453 ~ p.460
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Expression of HSP27 and αB-crystallin in Avian Cerebellum during Development
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Seo Je-Hoon
Moon Sang-Won
Sohn Hyun-Joon
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Abstract
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It is well known that small heat shock proteins play a role as molecular chaperone. However, during normal development of the cerebellum, expression and distribution of HSP27 and αB-crystallin (αBC) which are small heat shock proteins have not been reported. To verify the protective role of HSP27 and αBC in neurons and glial cells, we examined the expression and distribution of HSP27 and αBC in the developing chick cerebellum using immunoblot, immunohistochemical and double immunofluorescence staining. Expression of both HSP27 and αBC was first identified in the cerebellum of the embryonic day 14 (E14) embryo, and was increased at E18. Double immunofluorescence analysis with myelin-basic protein (MBP) demonstrated that αBC positive (+) cells were mature myelinating oligodendrocytes. αBC+ cells were observed in the white matter of the E14 cerebellum. At E18, there were a number of αBC+ cells in the white matter and a few cells in the granular layer of the gray matter. On the other hand, HSP27+ cells were observed in the white matter and the Purkinje cell layer at E14. At E18, HSP27+ signals were observed in Purkinje cells and neurons of cerebellar nucleus as well as oligodendrocytes in the white matter and the granular layer. The results that HSP27 and αBC were expressed in specific neurons and glial cells in the developing cerebellum suggest that HSP27 and αBC may be involved in the protective mechanism for the apoptosis of neurons and the physiological stress occurred in oligodendrocyts during cell maturation.
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KEYWORD
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Small heat shock protein, HSP27, αB-crystallin, Developing cerebellum, Purkinje cel, Oligodendrocyte, Cerebellar nucleus
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